热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
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热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：DOBR00 规格：96T
Cell Culture Supernates
Serum, EDTA Plasma, Heparin Plasma
The recovery of human Leptin sR spiked to levels throughout the range of the assay in various matrices was evaluated.
Human Leptin R Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.128 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Leptin sR
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Leptin sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Leptin sR in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Leptin R/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Leptin sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Leptin sR.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
1.83
5.15
11.2
1.69
5.09
10.7
Standard Deviation
0.071
0.283
0.292
0.097
0.282
0.633
CV%
3.9
5.5
2.6
5.7
5.5
5.9
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
2.34
7.01
14.6
2.34
6.95
14.3
Standard Deviation
0.142
0.155
0.709
0.202
0.37
0.966
CV%
6.1
2.2
4.9
8.6
5.3
6.7
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
96
91-102
EDTA Plasma (n=4)
104
92-115
Heparin Plasma (n=4)
103
92-113
Serum (n=4)
106
95-114
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Leptin sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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