热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
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热点关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 幼鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
货号：DTFP10 规格：96T
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Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
The recovery of TFPI spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Urine (50 uL)
Sensitivity
6.7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human TFPI. This kit recognizes predominantly free TFPI and a very small percentage of the LDL- and HDL-bound TFPI.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human TFPI Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human TFPI in cell culture supernates, plasma, and urine. It contains NS0-expressed recombinant human TFPI and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFPI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFPI.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
299
554
1108
301
569
1127
Standard Deviation
13.5
22.2
40.3
18.5
30.6
66.2
CV%
4.5
4
3.6
6.1
5.4
5.9
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
92
85-100
Urine (n=4)
94
85-106
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TFPI were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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